Bondy-Denomy Lab - Associate Specialist SCHOOL OF MEDICINE - Microbiology & Immunology
Description The Bondy-Denomy lab is seeking an Associate Specialist to join our group and contribute to new and ongoing projects. Our lab focuses on CRISPR-Cas immune systems and their natural functions in bacteria. We are also interested in developing new tools stemming from both Type I and II CRISPR-Cas systems, both for inhibitor proteins and new applications for these systems in microbes (see Bondy-Denomy et al., Nature 2015, Rauch et al., Cell 2017). The candidate will be expected to contribute to this tool development work in the lab, by conducting microbiology and molecular biology techniques, including bacterial and bacteriophage manipulation, cloning, and protein expression. An exceptional candidate will be able to design and execute gene editing experiments in bacterial and mammalian cells, measure the outcomes, and adjust parameters accordingly. Additionally, the specialist (like all members of the lab) will be expected to assist with managing day-to-day lab operations, and perform general lab duties and be a positive lab citizen.
Required Qualifications: • PhD degree in molecular genetics, microbiology, or related discipline is required. • Two or more years of recent, directly related laboratory work experience utilizing techniques or methods required by the position. • Experience with molecular and cellular biology techniques (including DNA and RNA isolation, PCR, gel electrophoresis, DNA assembly, bacterial transformation, mammalian cell culture, etc.). • Extensive experience in microbiology techniques (culturing, aseptic technique, plasmid manipulation, generating deletion mutants, DNA/RNA extractions) • Excellent organizational and interpersonal communication skills (verbal and written). • Willingness and ability to learn new methods and skills for changing research priorities. • Ability to work independently and as a member of a research team. • Ability to prioritize tasks, coordinate work tasks with others, and meet multiple deadlines.
Preferred Qualifications: • Prior experience with handling pathogenic microbes, aseptic techniques. • Prior experience in the design and assembly of DNA constructs. • Prior experience in bacterial genome manipulation (i.e. gene knockouts, locus removal, introduction of chromosomal tags and point mutations) and high throughput sequencing.
We are seeking at least a 1-year commitment. Depending on performance and workload, opportunities for independent research, mentorship of other trainees, and scientific writing will be available.
UC San Francisco seeks candidates whose experience, teaching, research, or community service has prepared them to contribute to our commitment to diversity and excellence.
The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status.
The University of California, San Francisco (UCSF) is a leading university dedicated to promoting health worldwide through advanced biomedical research, graduate-level education in the life sciences and health professions, and high-quality patient care. It is the only UC campus in the 10-campus system dedicated exclusively to the health sciences.