The Specialist will be involved in Neural cancer research. The Specialist will perform molecular biology techniques, particularly cloning targeting constructs and CRISPR plasmids. The Specialist will assist in culturing and differentiating human stem cells and analyzing protein expression via flow cytometry, immunofluorescence, western blot, and assessing cell viability. The Specialist will also work with mice, performing surgeries and orthotopic injections and isolate tumors from the mice. The Specialist will have the opportunity to present at lab meetings and will assist the lab manager with related duties.
Key responsibilities include: • The Specialist will assist in culturing and differentiating human stem cells. • The Specialist will assist in Lab Manager duties (lab ordering, fixing equipment). • The Specialist will perform molecular biology techniques (lentivirus production, genome editing, cloning), particularly cloning targeting constructs and CRISPR plasmids. • The Specialist will perform protein analysis. • The Specialist will perform mouse work (e.g. genotyping, surgeries, harvesting tumors).
Required qualifications: • BA/BS in a related science and two or more years of recent, directly related work experience following degree completion, utilizing the techniques or methods required by the position. • Previous laboratory experience. • Demonstrates a passion for science. • Ability to work independently in a team environment, good organizational skills.
Preferred qualifications: • Previous experience in differentiating human stem cells toward neural crest cells. • Previous experience in creating and analyzing tools for genome engineering (e.g. CRISPR/Cas9, TALEN). • Previous experience in performing mouse intracranial surgeries. • Previous experience working with human pluripotent stem cells.
Appointees in the Specialist series will be expected to engage in specialized research, professional activities and do not have teaching responsibilities. Specialists are expected to use their professional expertise to make scientific and scholarly contributions, and may participate in University and Public Service. Screening of applicants will begin immediately and will continue as needed throughout the recruitment period. Salary and rank will be commensurate with the applicants experience and training.
UC San Francisco seeks candidates whose experience, teaching, research, or community service that has prepared them to contribute to our commitment to diversity and excellence. The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status.
The University of California, San Francisco (UCSF) is a leading university dedicated to promoting health worldwide through advanced biomedical research, graduate-level education in the life sciences and health professions, and high-quality patient care. It is the only UC campus in the 10-campus system dedicated exclusively to the health sciences.