The Bondy-Denomy lab is seeking a Junior Specialist to join our group and contribute to new and ongoing projects. Our lab focuses on the interactions between bacteria and their viruses, with a specific interest in CRISPR-Cas immune systems. Our interest in this host-pathogen interaction has led to the discovery of numerous proteins that inhibit CRISPR-Cas function (Bondy-Denomy et al., Nature 2015, Rauch et al., Cell 2017). We are interested in further discovery and mechanistic characterization of these proteins to further our understanding of the molecular arms race in the microbial world. We also seek to develop new applications for enhancing the accuracy of CRISPR-Cas gene editing technology. The candidate will be expected to contribute to this work in the lab, by conducting microbiology and molecular biology techniques, including bacterial and bacteriophage manipulation, cloning, and protein expression. An exceptional candidate will design and execute gene editing experiments in mammalian cells. Additionally, the specialist will be expected to manage day-to-day lab operations, and perform general lab duties including equipment maintenance, preparation of stock solutions, plates, and media.
Required Qualifications • BA/BS degree may be in process at the time of application submission, however, the degree must be possessed by the appointment effective date. • One or more years of recent, directly related laboratory work experience utilizing techniques or methods required by the position. • Experience with general laboratory techniques, especially basic molecular and cellular biology techniques (including but not limited to DNA and RNA isolation, PCR, gel electrophoresis, DNA assembly, bacterial transformation, mammalian cell culture, etc.). • Basic microbiology techniques (culturing, generating deletion mutants) • Excellent organizational and interpersonal communication skills (verbal and written). • Willingness and ability to learn new methods and skills for changing research priorities. • Ability to work independently and as a member of a research team. • Ability to prioritize tasks, coordinate work tasks with others, and meet multiple deadlines.
Preferred Qualifications • Prior experience with handling pathogenic microbes, aseptic techniques • Prior experience in the design and assembly of DNA constructs • Prior experience with mammalian cell culture
We are seeking at least a 1-year commitment. Depending on performance and workload, opportunities for independent research, mentorship of other trainees, and scientific writing will be available. This position is ideal for someone seeking research experience towards the pursuit of graduate school or medical school.
UC San Francisco seeks candidates whose experience, teaching, research, or community service has prepared them to contribute to our commitment to diversity and excellence. The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status.
The University of California, San Francisco (UCSF) is a leading university dedicated to promoting health worldwide through advanced biomedical research, graduate-level education in the life sciences and health professions, and high-quality patient care. It is the only UC campus in the 10-campus system dedicated exclusively to the health sciences.