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Application Review Date The First Review Date for this job is: April 18, 2019
Departmental Overview The Wilson lab is dedicated to development of new technologies enabling therapeutic genome editing. To do this, two key resources are essential: highly pure genome-editing enzymes, as well as made-to-order cell lines for use as reporters in genome-editing assays. To this end, we are seeking a skilled Specialist capable of producing bespoke proteins and cell lines to support Wilson lab research efforts.
Cloning & purification of made-to-order genome-editing enzyme samples.
Complete focused, journey-level projects in the distinct fields of protein engineering and cellular engineering.
Generation and design of novel reporter cell lines for genome editing assays.
Validation & QC of above research assets via techniques such as gel electrophoresis, FPLC-based chromatography, mass spectrometry, immunohistology, flow cytometry, and DNA analysis.
Develop protocols for efficient production of research assets and characterize and validate these assets upon their production.
Training of mentees in established research protocols.
Keep track of work schedule, paperwork, sample storage, and inventory), including use of electronic/shared laboratory notebook systems (e.g. Benchling).
Adhere to all campus and EH&S safety policies.
Note-taking during labs as well as data maintenance using spreadsheet software, Co-author manuscripts, and other duties as assigned.
Extensive experience with molecular cloning, recombinant protein expression (including knowledge of various helper cell lines & plasmids), and protein purification (including use of various FPLC-based chromatography approaches: affinity, ion exchange, size exclusion).
Experience with cell-line generation including lentiviral production/transduction, single-cell sorting to produce clonal lines, and characterization via immunohistology.
Ability to work on multiple assignments, set priorities, reprioritize with ease mid-task, and take initiative in defining and executing work. Ability to work independently as well as collaboratively on a team.
Excellent interpersonal communication skills, especially regarding technical details, instrumentation, etc.
Excellent organization skills (General computer skills (Microsoft Word, PowerPoint, Excel, cloud-based Google apps) for keeping track of results and sharing progress, including use of electronic laboratory inventory systems.
Experience with standard molecular biology techniques: PCR; electrophoresis; western blot; microscopy.
Experience with cellular reporters of genome-editing (e.g. GFP on/off, GFP-to-BFP, etc.).
Experience with cellular reporters of intracellular trafficking (e.g. split GFP systems to assay for endosomal escape).
Experience with fluorescent microscopy/histology of live or fixed cells.
Flow cytometry for cell characterization and/or single-cell sorting.
Experience with endotoxin removal from bacterially expressed recombinant proteins.
Performing such tasks independently requires expert-level skills including but not limited to: familiarity with current best-in-practice cloning strategies, awareness of structure/function relationships of genome-editing enzymes such as Cas9, and protein engineering best practices such as the impact of incorporating fusion domains and/or codon optimization.
Extensive experience in protein biochemistry, including expert knowledge of molecular cloning and FPLC-based purification
Substantial experience in cell engineering, including assembly of reporter constructs, generation and use of lentiviral vectors, generation of clonal cell populations, and characterization of said clonal cell lines.
Minimum 3 years of post-graduate research experience.
Salary & Benefits This an exempt, monthly paid position. Salary rate will be commensurate with experience.
For information on the comprehensive benefits package offered by the University visit:
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