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Application Review Date The First Review Date for this job is: April 26, 2019
Departmental Overview The California Institute for Quantitative Biosciences (QB3) is a cooperative effort between the state of California, private industry, venture capital, and the University of California campuses at Berkeley, San Francisco, and Santa Cruz. In order to stimulate discovery at the intersection of the physical and biological sciences, QB3 promotes multidisciplinary research at world-class facilities, creates innovative educational programs, and fosters industry partnerships. The Institute's more than 180 researchers decipher the complex systems involved in living systems and discover ground-breaking applications for that basic knowledge in the areas of health, energy, and the environment.
The Lareau lab in the Department of Bioengineering and the California Institute for Quantitative Biosciences at UC Berkeley has an opening for a Staff Research Associate I. Our research uses bioinformatics and molecular biology to understand post-transcriptional gene regulation. We make extensive use of genome-scale approaches and deep sequencing.
We are looking for a Staff Research Associate to play a central supporting role in the research program of the laboratory. The SRA will lead in the design, implementation and analysis of genome editing experiments as well as next-generation sequencing (NGS) and other molecular biology experiments. The SRA will also take the lead in purchasing equipment and reagents for our laboratory. The selected candidate should have good communication skills and the ability to work independently and design and analyze their own experiments.
Responsibilities The Staff Research Associate performs a variety of techniques in the field of molecular biology, including genome editing with CRISPR/Cas9, mammalian cell culture including stem cell culture, preparation of NGS libraries, analytical techniques such as quantitative real time PCR (qPCR), and molecular cloning of expression constructs.
Design and implement experiments to analyze post-transcriptional gene regulation
RNA isolation and generation of next-generation sequencing libraries
Troubleshoot problems with molecular biology workflows
Maintain mammalian cell cultures and design protocols for transfecting cells and collecting RNA and DNA
Perform genome editing with CRISPR/Cas9
Molecular cloning of expression constructs
Perform miscellaneous tasks required to operate the research laboratory (ordering supplies, maintaining equipment, etc).
Analyze data and independently determine the best directions for follow-up work.
The successful candidate will be motivated, team-oriented, pay strict attention to detail, keep industry-standard records and have good communication skills.
Must adhere to all campus and EH&S safety policies.
Familiarity with general molecular biology methods, such as ligation, gene cloning, gel electrophoresis of nucleic acids, PCR, and western blotting.
Experience with mammalian cell culture and genome editing.
B.S. or B.A. degree in the biological sciences or a related field with demonstrated success in general molecular biological techniques or equivalent experience.
Previous experience working in research laboratories.
Experience in preparation of short read DNA libraries, RNA molecular biology techniques, fluorescent quantitation of DNA samples, and real time (qPCR) PCR.
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