About Berkeley The University of California, Berkeley, is one of the world's most iconic teaching and research institutions. Since 1868, Berkeley has fueled a perpetual renaissance, generating unparalleled intellectual, economic and social value in California, the United States and the world. Berkeley's culture of openness, freedom and acceptanceacademic and artistic, political and culturalmake it a very special place for students, faculty and staff.
Berkeley is committed to hiring and developing staff who want to work in a high performing culture that supports the outstanding work of our faculty and students. In deciding whether to apply for a staff position at Berkeley, candidates are strongly encouraged to consider the alignment of the Berkeley Workplace Culture with their potential for success at http://jobs.berkeley.edu/why-berkeley.html.
Application Review Date The First Review Date for this job is: May 30, 2019.
Departmental Overview The California Institute for Quantitative Biosciences (QB3) is one of four Governor Gray Davis Institutes for Science and Innovation established in 2000 to ensure the future of the California economy by promoting research and innovation. QB3 is a cooperative effort between the state of California, private industry, venture capital, and the University of California campuses at Berkeley, San Francisco, and Santa Cruz.
QB3 harnesses the quantitative sciences of physics and engineering to unify our understanding of biological systems at all levels of complexity, from atoms and molecules to cells, tissues, and entire living organisms. QB3 scientists make discoveries that drive the development of technologies, products, and wholly new industries, ensuring that California remains competitive in the 21st century.
QB3's goals are to fuel the California bioeconomy; to support research and training in quantitative biosciences; and to translate academic research into products and services that benefit society.
Responsibilities Protein purification:
Protein expression in E. coli and yeast, from small-scale expression testing to large-scale expression for bulk purifications.
Purification of recombinant proteins using defined protocols on automated FPLC chromatography systems.
Optimization of these protocols as needed, based (in part) on literature and database research.
Maintain FPLC systems in good working order.
Maintain stocks of in-house purified enzymes.
Initiate projects with customers based on their needs and expectations, plan and execute project work with clearly defined goals and time-frames.
Keep accurate records and prepare clear concise reports for customers.
DNA Cloning/Molecular Biology:
Assist staff in maintaining stocks of competent cells and other molecular biology reagents.
Construction of DNA plasmids using a range of cloning techniques.
Analysis of sequencing data.
Perform all procedures in accordance with NIH recombinant DNA guidelines.
Assist in ordering and stocking common lab items, and in the general upkeep of the laboratory.
Contribute to lab safety.
Contribute to the development of additional services and the improvement of existing ones.
Bachelor or Master's degree in biochemistry, molecular biology or related field and /or equivalent experience.
At least 1 year experience with protein expression and purification.
Experience with FPLC chromatography systems (e.g. AKTA/NGC), including method creation and optimization, troubleshooting and maintenance.
Knowledge of sterile technique and essential methods of molecular biology and biochemistry.
Experience with standard DNA cloning techniques.
Proficiency in using computers for writing and keeping spreadsheets.
Ability to work independently as well as cooperatively as a team member.
Attention to detail and excellent communication skills.
Experience with basic CRISPR methods.
Experience with protein expression in insect cell culture.
How to Apply Please submit your cover letter and resume as a single attachment when applying.
Conviction History Background This is a designated position requiring fingerprinting and a background check due to the nature of the job responsibilities. Berkeley does hire people with conviction histories and reviews information received in the context of the job responsibilities. The University reserves the right to make employment contingent upon successful completion of the background check.
The University of California was chartered in 1868 and its flagship campus - envisioned as a "City of Learning" - was established at Berkeley, on San Francisco Bay. Today the world's premier public university and a wellspring of innovation, UC Berkeley occupies a 1,232 acre campus with a sylvan 178-acre central core. From this home its academic community makes key contributions to the economic and social well-being of the Bay Area, California, and the nation.