Junior/Assistant/Associate/Full Specialist (Protein Engineering) Search# JPF02520 Antibiome Center, Department of Pharmaceutical Chemistry UCSF
The laboratory of Jim Wells and the Antibiome Center is seeking a highly motivated candidate to join our quick paced and multi-talented team. The successful candidate will be responsible for molecular biology, protein/antibody expression and purification, and protein/antibody validation. A steep learning curve is expected, and the candidate will have opportunities to learn state-of-the-art techniques. Our laboratory is highly collaborative and is engaged in numerous academic and industrial collaborative projects.
The Antibiome Center is part of the Recombinant Antibody Network (RAN), a collaborative consortium consisting of laboratories at the University of California San Francisco (Dr. Jim Wells), University of Chicago (Dr. Tony Kossiakoff), and the University of Toronto (Dr. Sachdev Sidhu). We are committed to generate recombinant antibodies at the surface proteome-wide scale using high throughput phage display approaches. Our long history of protein engineering expertise also enables the development of bio-therapeutic antibodies in the immuno-oncology field.
Primary responsibilities • Cloning recombinant protein and antibody construct using a variety of molecular biology techniques including Polymerase Chain Reaction, Gibson assembly, DNA purification, and transformation. • Protein expression from bacterial and mammalian sources. • Purification of proteins using chromatography. • Characterization of proteins by variety of techniques including SDS-PAGE, ELISA, SEC, and Western Blotting.
Required Qualifications • BS in biochemistry, cell biology, or relevant life sciences field with 1-2 years wet laboratory experience at the Junior Specialist rank. • MS or PhD in relevant life sciences field with at least five years of research experience for Assistant/Associate Specialist rank. • PhD in relevant life sciences field with at least ten years of research experience for Full Specialist rank. • Foundational understanding and experiences in molecular biology, recombinant protein expression, and purification techniques. • Candidates must meet the requirements by the time of hire.
Preferred Qualifications • Expertise with phage display library generation and phage selection. • Experience with Next-Generation-Sequencing techniques and analysis. • Familiar with mammalian tissue culture techniques including transfections, cell line maintenance, recombinant protein expression. • Proficiency with advance biophysical and structure characterization of proteins/antibodies using CD, DSF, mass spectrometry, SPR, BLI, negative stain EM, and X-ray crystallography. • Data analysis experience using programing languages such as R or Python.
Appointment and salary will be commensurate with the applicant's experience and training.
UC San Francisco seeks candidates whose experience, teaching, research, or community service that has prepared them to contribute to our commitment to diversity and excellence. The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status.
The University of California, San Francisco (UCSF) is a leading university dedicated to promoting health worldwide through advanced biomedical research, graduate-level education in the life sciences and health professions, and high-quality patient care. It is the only UC campus in the 10-campus system dedicated exclusively to the health sciences.