The research focus of Dr. Shlomo Melmed is molecular pathogenesis of pituitary tumors and pituitary receptor signaling. He employs molecular, cellular, transgenic animal and human tissue models to understand the biology of these commonly encountered hormone-secreting adenomas, and tests pituitary receptor signaling to develop novel peptide analog treatments for these tumors secreting growth hormone, prolactin, ACTH or gonadotropins. For more information, please visit https://www.cedars-sinai.org/research/labs/melmed.html .
** This is a one-year term position, ending December 2020 **
Working independently but in close cooperation and consultation with the Principal Investigator and other Research Scientists, the Postdoctoral Scientist will perform routine and complex laboratory procedures throughout training period. The Postdoc may develop, adapt, and implement new research techniques and protocols, analyze and interpret data, and participate in publications and presentations as author or co-author.
Additional Job Duties and Responsibilities:
May assist in preparation of grant proposals, but is not responsible for generating grant funds.
Designs and performs experiments and keeps appropriate experimental records and documentation; analyzes the results with the Principal Investigator.
Operates and maintains equipment and instruments.
Will investigate the mechanisms of pituitary tumorigenesis
Specific laboratory procedures conducted: plasmid construction, DNA/RNA/protein extraction, SDS-polyacrylamide gel electrophoresis, western blotting, immunoprecipitation, luciferase reporter gene assay, site-directed mutagenesis, Glutathione S-transferase (GST) pull-down experiments, PCR, RT-PCR, quantitative real-time PCR, ELISA, IgG purification, DNA digestion, DNA amplification, ligation and subcloning, DNA or siRNA transfection, direct sequencing, mammalian cell culture and transfection, blood and organ collection (from rats and mice), isolation of primary hepatocyte (rat), primary culture of anterior pituitary cells (mice), injection into tail vein of mice, in vivo knockdown experiment by antisense oligonucleotide
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