Eyquem Lab- Junior/Assistant/ Associate/ Full Specialist
We are recruiting a Junior/Assistant/Associate/Full Specialist to assist with ongoing multidisciplinary CAR T cell projects. Our lab is interested in engineering next generation CAR T cell using the CRISPR/Cas9 system to enhance their activity, specificity, persistence and ultimately improve their therapeutic activity against solid tumor. We recently showed (Eyquem et al. Nature 2017) that targeting the CAR transgene to a specific locus offers optimal CAR expression that prevents undesirable differentiation and exhaustion, which results in superior anti-tumor activity in hematological malignancies. These results greatly improved the understanding of CAR biology by establishing the first link between CAR expression and anti-tumor function. We are now applying these technologies to tackle solid tumors.
Working as part of a multidisciplinary group, the AS will utilize various protocols to generate the molecular components, such as DNA constructs, gRNA library, lentiviral vectors and Adeno-Associated Vectors necessary for specific cellular engineering experiments. Additional responsibilities will consist of isolation and culture of primary human T cells, as well as functional analysis of engineered cells via Sanger sequencing, next-generation DNA sequencing, Single-Cell RNA sequencing and flow cytometry. The AS’s role will also include the design and completion of cellular editing experiments, as well as in vivo analysis of novel cellular cancer immunotherapies.
Required Qualifications: • Specialist appointed at the Junior rank must possess a bachelor’s degree or at least 4 years of research experience. • Specialists appointed at the Assistant or Associate ranks must possess a master’s degree (or equivalent degree) or at least 5 years of research experience. • Specialists appointed at the Full rank must possess a terminal degree or ten or more years of experience. • Excellent organizational and interpersonal communication skills (verbal and written) • Willingness and ability to learn new methods and skills for changing research priorities. • Ability to work independently and as a member of a research team. • Ability to prioritize tasks, coordinate work tasks with others, and meet multiple deadlines.
Preferred Qualifications: • Master degree and with 3+ years laboratory experience utilizing techniques or methods required by the position; or an equivalent combination of education and experience. • Experience with general laboratory techniques, especially basic molecular and cellular biology techniques (including but not limited to DNA and RNA isolation, PCR, gel electrophoresis, DNA assembly, bacterial transformation, mammalian cell culture, etc.) • Design and cloning of CRSIPR library • Production of Lentiviral vector for gRNA library and transduction of cell lines or primary cells • Preparation of NGS library • Excellent organizational and interpersonal communication skills (verbal and written) • Willingness and ability to learn new methods and skills for changing research priorities • Ability to work independently and as a member of a research team • Ability to prioritize tasks, coordinate work tasks with others, and meet multiple deadlines
UC San Francisco seeks candidates whose experience, teaching, research, or community service that has prepared them to contribute to our commitment to diversity and excellence. The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status.
The University of California, San Francisco (UCSF) is a leading university dedicated to promoting health worldwide through advanced biomedical research, graduate-level education in the life sciences and health professions, and high-quality patient care. It is the only UC campus in the 10-campus system dedicated exclusively to the health sciences.