UCSD Layoff from Career Appointment: Apply by 01/30/2020 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 02/10/2020. Eligible Special Selection clients should contact their Disability Counselor for assistance.
Perform tumor immunology studies consisting of murine models of cancer, animal breeding and genotyping, flow cytometry, bone marrow transplantation, immunohistochemistry, in vitro cell biology, and in vitro biochemistry. Murine tumor biology studies will include xenograft, orthotopic and spontaneous tumor growth and metastasis assays, immunological analysis, treatment of animals with experimental therapeutic agents by gavage, intravenous and intraperitoneal methods, breeding and genotyping of transgenic and mutant mice, bone marrow transplantation and immune cell isolations. Perform major surgery, including small animal anesthesia.
In addition, perform immunohistochemistry of frozen and fixed tissues or cells. Conduct various in vitro cell biology techniques to include performing flow cytometry analysis of cell surface protein expression, cell adhesion and migration assays, transfecting cells with expression constructs, preparing lysates of cells in culture or tissues, quantification, ELISA assays, SDS PAGE, Western and Immunoblotting, phosphorylations, and gel electrophoresis. Additionally, perform recombinant DNA techniques to include preparing plasmids and maintaining plasmid stocks, analyzing DNA and RNA purification, and conducting DNA electrophoresis, and RT-PCR.
Make innovative contributions regarding experimental design and methodology, using published scientific data in referred journals, as well as online using search engines and subject-specific websites, to make recommended changes to current protocols and practices. Troubleshoot problems for quality control on immunochemical and histochemical procedures. Maintain and prepare accurate records of all experiments. Assist in the preparation of data for publication. Assume responsibility for lab maintenance and safety training. Provide training and work direction in areas of expertise as needed. Maintain and prepare accurate records of all experiments. Assist in the preparation of data for publication.
Advanced theoretical knowledge in molecular biology, biochemistry, physiology and cell biology.
Demonstrated experience in mouse handling, breeding, genotyping, surgery, immunology and tumor growth and metastasis assays, including performing flow cytometry as well as immunohistochemistry.
Proven experience with immunology, cell biology, and protein biochemistry techniques; including flow cytometry, ELISA assays, SDS PAGE, Western and Immunoblotting, phosphorylations and gel electrophoresis, cell migrations assays, cell-substrate adhesion assays, cell-cell adhesion assays, ELISA, immunocytochemistry and cell transfection.
Thorough experience and knowledge in molecular biology, including preparation and maintenance of virus and plasmid stocks; performing DNA and RNA purification, DNA electrophoresis and RT-PCR.
Experience managing laboratory environment, including ordering, safety management and maintenance of basic laboratory equipment, such as incubators, centrifuges, pipetters, spectrophotometers, balances pH meters, fluorescence microscopes, water baths, and electrophoresis equipment.
Demonstrated knowledge in making innovative contributions to experimental design and methodology by reviewing published scientific data in refereed journals.
Strong communication skills (both verbal and written) needed to interact professionally and effectively in the work environment, as well as to effectively and accurately document research procedures. Ability to read, comprehend, and discuss research materials.
Demonstrated ability to design, develop and implement new research and experimental techniques to meet research objectives.
Ability to follow established experimental protocols with accuracy and efficiency and to design, develop and implement new research and experimental techniques to meet research objectives.
Employment is subject to a criminal background check.
Must be able to work occasional evenings or weekends if needed.
Proven ability to work with animals and willingness to work with bioharzardous materials.
UC San Diego is an academic powerhouse and economic engine, recognized as one of the top 8 public universities by U.S. News and World Report. Innovation is central to who we are and what we do. Here, students learn that knowledge isn't just acquired in the classroom - life is their laboratory. UC San Diego's rich academic portfolio includes six undergraduate colleges, five academic divisions and five graduate and professional schools. The university's award-winning scholars are experts at the forefront of their fields with an impressive track record for achieving scientific, medical and technological breakthroughs.