Pediatrics Hematology/Oncology - Project Scientist series University of California Los Angeles
Requisition Number: JPF05878
The Division of Hematology-Oncology in the Department of Pediatrics of the David Geffen School of Medicine at UCLA is seeking a Project Scientist. The appointment will be in the Project Scientist series; academic rank will be commensurate with experience. This position requires a Ph.D. or equivalent and prior experience. This is a Non-Senate position.
(Manufacturing products for clinical research according to Good Manufacturing Practice (GMP) guidelines
Operate in a GMP regulated environment according current standard operating protocols
Meticulous record keeping and maintenance of GMP, GTP and basic research related records and documents.
Attend and arrange seminars, training sessions and workshops to maintain competency in evolving current GMP/GTP regulations
Performing post-manufacturing lot-release tests: Elisa, Colony-forming Unit (CFU) assay, ELISA, Endotoxin assay, Mycoplasma assay, vector copy number (VCN) test by ddPCR
Culture of hematopoietic progenitor cells for in vitro differentiation into T-cells for proof of principle testing of gene delivery
Conduct experiments for FDA IND application(s)
Conduct DNA, RNA and protein isolation and purification according current GLP research grade protocols
Quantification of nucleic acids and proteins
Perform PCR and qPCR
Perform multiparametric flow cytometry to assist on-going specific NYESO-TCR research project for intracellular, surface and peptide target markers.
Culture adhered and non-adherent mammalian cells, preparing media, aliquots and other compounds utilized in tissue culture with proper sterile aseptic technique.
Ability to set up co-cultures for killing-assays or peptide release production-analysis
Ability to set up experiments utilizing real time in vitro cell imaging system Incucyte
Perform ELISA experiments in either cellular supernatants or cellular lysates normalizing results against total protein content
Plasmid preparation, bacteria growth on competent E. coli or Stable 3 systems, positive transformants selection and sequences analysis
Ability of align sequences on APE-software (or-equivalent) and design plasmids for plasmid-vector construction and PCR analysis
Perform plasmid sequencing and re-sequencing
Production of high-titer viral vectors, purification and concentration of viral supernatants for retrovirus
Production of high-titer viral vectors, purification and concentration of viral supernatants for lentivirus
Determination of viral titer by ddPCR on fresh and frozen supernatants
Document results of experiments and elaboration of reports for NYESO-TCR team leader
Perform experiments associated to survival tittering and transduction efficiency on target cells
Design and re-design of viral vectors for large scale manufacture of GMP and GMP-comparable clinical grade virus.
The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status. For the complete University of California nondiscrimination and affirmative action policy see: UC Nondiscrimination and Affirmative Action Policy http://policy.ucop.edu/doc/4000376/NondiscrimAffirmAct.
The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status. For the complete University of California nondiscrimination and affirmative action policy, see: UC Nondiscrimination & Affirmative Action Policy, https://policy.ucop.edu/doc/4000376/DiscHarassAffirmAction
UCLA is known worldwide for the breadth and quality of its academic, research, health care, cultural, continuing education and athletic programs. UCLA offers undergraduate degrees in more than 127 majors and graduate degrees in 198 program areas. UCLA has 11 highly regarded professional schools. Eight are ranked among the nation's top 15 in their field by U.S. News & World Report. UCLA is consistently among the most popular campus in the nation for undergraduate applicants. The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to age, race, color, religion, sex, national origin, disability, or protected veteran status. Benefits:UCLA offers a comprehensive benefits package, including an average of three weeks' vacation per year; an average of 12 days per year sick leave; 13 paid holidays per year; health, dental and optical benefits; life insurance; disability insurance; the University of California Retirement Plan with 5 year vesting and various voluntary UC Savings Plans. There are also special programs and privileges available, such as accessibility to cultural and recreational programs, athletic events, and the University Credit Union.