At the University of California, Berkeley, we are committed to creating a community that fosters equity of experience and opportunity, and ensures that students, faculty, and staff of all backgrounds feel safe, welcome and included. Our culture of openness, freedom and belonging make it a special place for students, faculty and staff.
The University of California, Berkeley, is one of the world's leading institutions of higher education, distinguished by its combination of internationally recognized academic and research excellence; the transformative opportunity it provides to a large and diverse student body; its public mission and commitment to equity and social justice; and its roots in the California experience, animated by such values as innovation, questioning the status quo, and respect for the environment and nature. Since its founding in 1868, Berkeley has fueled a perpetual renaissance, generating unparalleled intellectual, economic and social value in California, the United States and the world.
We are looking for equity-minded applicants who represent the full diversity of California and who demonstrate a sensitivity to and understanding of the diverse academic, socioeconomic, cultural, disability, gender identity, sexual orientation, and ethnic backgrounds present in our community. When you join the team at Berkeley, you can expect to be part of an inclusive, innovative and equity-focused community that approaches higher education as a matter of social justice that requires broad collaboration among faculty, staff, students and community partners. In deciding whether to apply for a position at Berkeley, you are strongly encouraged to consider whether your values align with our Guiding Values and Principles, our Principles of Community, and our Strategic Plan.
Application Review Date
The First Review Date for this job is: November 18, 2020
The California Institute for Quantitative Biosciences (QB3) is a cooperative effort between the state of California, private industry, venture capital, and the University of California campuses at Berkeley, San Francisco, and Santa Cruz. In order to stimulate discovery at the intersection of the physical and biological sciences, QB3 promotes multidisciplinary research at world-class facilities, creates innovative educational programs, and fosters industry partnerships. The Institute's more than 180 researchers decipher the complex systems involved in living systems and discover ground-breaking applications for that basic knowledge in the areas of health, energy, and the environment.
Under the supervision of the Macrolab Director and in collaboration with current staff, will provide essential all-round support to the facility. The position will: 1) interact with facility customers, 2) plan customer projects, 3) carry out and document technical lab work, 4) contribute to day-to-day laboratory duties and organization.
Laboratory work will be mainly focused on protein expression and purification, using established protocols and optimizing or developing new ones as needed, but will also include DNA cloning and other molecular biology techniques. The applicant must have the organizational and interpersonal skills to interact with customers, and demonstrate sufficient independence to perform routine tasks and established protocols with minimal supervision in order to keep the laboratory running at a high level of productivity.
Initiate projects with customers, and with minimal supervision, develop an appropriate protocol for the project.
Provide guidance to customers if necessary to meet their needs.
Define the goals and timelines for the project and keep customers and Director updated with progress reports.
Purification of recombinant proteins using defined protocols on automated FPLC chromatography systems.
Optimization of these protocols as needed, based (in part) on literature and database research.
Maintain FPLC systems in good working order.
Maintain stocks of in-house purified enzymes.
Keep accurate records and prepare clear concise reports for customers.
DNA CLONING/MOLECULAR BIOLOGY
Assist staff in maintaining stocks of competent cells and other molecular biology reagents.
Construction of DNA plasmids using a range of cloning techniques.
Analysis of sequencing data.
Perform all procedures in accordance with NIH recombinant DNA guidelines.
Assist in ordering and stocking common lab items, and in the general upkeep of the laboratory.
Contribute to lab safety.
Contribute to the development of additional services and the improvement of existing ones.
Bachelor or Master's degree in biochemistry, molecular biology or related field and/or equivalent years experience or training and at least 1 year experience with protein expression and purification from E. coli.
Experience with FPLC chromatography systems (e.g. AKTA/NGC), including method creation and optimization, troubleshooting and maintenance.
Thorough understanding of the principles of column chromatography techniques for protein purification (including affinity, ion-exchange, size-exclusion).
Knowledge of sterile technique and essential methods of molecular biology and biochemistry.
Experience with standard DNA cloning techniques.
Proficiency in using computers for writing and keeping spreadsheets.
Ability to work independently as well as cooperatively as a team member.
Attention to detail and excellent communication skills.
Experience making chemically competent cells.
Experience with basic CRISPR methods.
Experience with protein expression in insect cell culture.
Salary & Benefits
Salary is commensurate with experience. Hiring range is $58,454 - $63,262/annually.
For information on the comprehensive benefits package offered by the University visit:
Please submit your cover letter and resume as a single attachment when applying.
This is a full-time career position which is governed by the terms and conditions in the agreement for the Research Support Professionals Unit (RX) between the University of California and the University Professional and Technical Employees (UPTE). The current bargaining agreement manual can be found at: http://ucnet.universityofcalifornia.edu/labor/bargaining-units/rx/index.html
Conviction History Background
This is a designated position requiring fingerprinting and a background check due to the nature of the job responsibilities. Berkeley does hire people with conviction histories and reviews information received in the context of the job responsibilities. The University reserves the right to make employment contingent upon successful completion of the background check.
Equal Employment Opportunity
The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or protected veteran status. For more information about your rights as an applicant see:
The University of California was chartered in 1868 and its flagship campus - envisioned as a "City of Learning" - was established at Berkeley, on San Francisco Bay. Today the world's premier public university and a wellspring of innovation, UC Berkeley occupies a 1,232 acre campus with a sylvan 178-acre central core. From this home its academic community makes key contributions to the economic and social well-being of the Bay Area, California, and the nation.