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Job posting will remain open until position is filled.
Under general supervision, the incumbent will be responsible for undertaking research projects in collaboration with the Principal Investigator (PI) making independent contributions to research and experiments regarding tissue culture of mammalian cell lines.
The incumbent will work independently to make determinations and design experiments in yeast work, which will include yeast strain genetic manipulation, yeast transformation of DNA and selection on the appropriate yeast cell plates, preparing yeast cell extracts for various biochemical analyses, immunofluorescence, live cell imaging and RNA analyses. In addition, the incumbent will grow established mammalian cell lines and primary mouse cells to generate protein extracts or nucleic acid (both DNA and RNA) or immunofluorescence or live-cell imaging samples. Furthermore, the incumbent will generate frozen mammalian cell lines and yeast strain stocks. Mammalian cell work will also include standard tissue culture cell maintenance, transfection of plasmid DNA, and selection and cloning of the appropriate transformants on the selectable media.
Other duties include: read and abstract articles of value in the prosecution of broad research problems; analyze and interpret genetic screens. Incumbent will participate in writing and publishing manuscripts in international journals, and attend and present research results at laboratory meetings. Finally, incumbent will provide work direction to laboratory staff, undergraduate and graduate students in overseeing lab databases of strains, cell lines and reagents. Perform other duties as assigned.
Strong theoretical and practical knowledge of biochemistry, molecular biology, immunology and genetics, including relevant laboratory techniques, acquired through a Bachelor's in Biology or related field OR an equivalent combination of education and laboratory experience. Master's or PhD preferred.
Proven ability to independently participate in the execution of research projects or portions of such projects, independently planning, organizing and performing experiments and selecting data for analysis.
Demonstrated experience with experimental techniques including but not limited to working with various types of fluorescent microscopes, molecular biology, biochemistry, genetics, sub-cloning to generate various forms of knockout and tagged genes.
Proven ability to independently utilize scientific literatures, online databases and other relevant resources to apply to research projects and execute experiments. Knowledge of PubMed or library literature search to identify relevant research papers, journals and books
Demonstrated ability to develop, follow and trouble-shoot established lab protocols and lab procedures with limited direction from PI.
Demonstrated ability to prepare and edit scientific papers, manuscripts, and lab data. Experience presenting research to formal audience.
Demonstrated successful, positive interpersonal communication skills needed to interact well with others to facilitate communication about lab requirements, including the ability to discuss and understand day-to-day laboratory affairs, research plans, results and ideas with PI and other personnel in a constructive, professional and pleasant manner.
Excellent organizational skills including the ability to keep clear and accurate records, organize a varied workload, follow written and oral protocols and give oral presentations on the progress of research in lab meetings. Proven ability to prioritize and schedule experiments and duties to maximize efficiency.
Familiarity with Apple and PC computers and associated word processing and spreadsheet software with a willingness and ability to update and increase skills and knowledg
UC San Diego is an academic powerhouse and economic engine, recognized as one of the top 8 public universities by U.S. News and World Report. Innovation is central to who we are and what we do. Here, students learn that knowledge isn't just acquired in the classroom - life is their laboratory. UC San Diego's rich academic portfolio includes six undergraduate colleges, five academic divisions and five graduate and professional schools. The university's award-winning scholars are experts at the forefront of their fields with an impressive track record for achieving scientific, medical and technological breakthroughs.