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Biomedical Technician - Applied Genomics, Computation & Translational Core

Cedars-Sinai Medical Center

Details

Posted: 17-Sep-23

Location: Los Angeles, California

Type: Full-time

Salary: Open

Internal Number: HRC1374248

The Applied Genomics, Computation & Translational Core is looking for a Biomedical Technician to join the team!

The Cedars-Sinai Applied Genomics, Computation, and Translational Core (AGCT Core) is a fully equipped, state-of-the-art genomics facility offering data generation and interpretation for basic science and translational research in next-generation sequencing technologies, including single cell omics, spatial transcriptomics, and bulk cell omics. We specialize in single cell omics and spatial transcriptomics via the 10x Genomics, Parse Biosciences, Mission Bio, and NanoString platforms. Our R&D team offers sample preparation for single cell assays, such as nuclei isolation from frozen tissue or single cell isolation from fresh tissue dissociation. The AGCT Core also offers comprehensive services for standard bulk cell sequencing in genomics, transcriptomics, epigenomics, and metagenomics. To learn more please visit Applied Genomics, Computation & Translational Core | Cedars-Sinai.

Are you ready to be a part of breakthrough research?

The Genomics Core Biomedical Technician participates in wet- and dry- laboratory projects and may execute tasks requiring compliance with all Federal and State accrediting agency requirements and/or Good Laboratory Practices (GLP). Responsible for assigned wet lab work, lab maintenance, and writing and recording controlled documents. Performs all activities strictly according to SOPs, with accurate documentation, and may assist quality control team with required activities. Installs and maintains laboratory equipment to ensure proper functioning of devices. Assists in addressing complex issues and troubleshooting.

Primary Duties and Responsibilities:

Performs a variety of project-specific process development tasks for multiple clients following standard operating procedures (SOPs).
Maintains good documentation in laboratory notebooks, data record/transfer, SOPs, and batch records.
Drafts standard operating procedures and maintains computer database.
Verifies samples received, logs samples into systems, and may transport to appropriate environment within the facility for storage following SOPs.
Performs lab maintenance duties, including glassware cleaning and sterilization.
Maintains lab equipment and related records.
Prepares cGMP production room, maintains material inventories, and places orders for equipment and supplies.
Assists in the operation of specialized equipment and machinery.
Ensures all activities comply with regulatory guidelines and safety standards, as appropriate.

Department-Specific Responsibilities:

Perform all aspects of cutting-edge NGS technology genomics experiments, including fluorescence-based nucleic acid quantification, Bioanalyzer and TapeStation fragment analysis, Illumina sequencing-by-synthesis, PacBio Single Molecule Real Time sequencing, bulk cell and single cell library preparation, spatial transcriptomics library preparation, and sample preparation.
Operation of Next-Generation Sequencing (NGS) sequencers including the Illumina NovaSeq 6000, NextSeq 500, and MiSeq, and the PacBio Sequel.
Operation of automated liquid handlers including the Hamilton Vantage and 10x Genomics Chromium Connect.
Operation of single cell sequencing platforms including the 10x Genomics Chromium and Mission Bio Tapestri systems.
Operation of Quality Control equipment including the Agilent Bioanalyzer and TapeStation, Invitrogen Qubit systems, and the Thermo Fisher Scientific NanoDrop 8000.
Operation of equipment used in sample preparation including the Miltenyi gentleMACS, autoMACS Pro Separator, MACSQuant Analyzer, and MACSQuant Tyto, S2 Genomics Singulator, Nexcelom Bioscience Cellaca, Thermo Fisher Scientific EVOS M7000.
Construction of bulk cell NGS libraries using commercially available reagent kits from Illumina, Integrated DNA Technologies, Takara Bio, Qiagen, Lexogen, and New England Biolabs.
Single cell capture and library construction using the 10x Genomics, Mission Bio, and Parse Biosciences platforms.
Liaise with post-doctoral, doctoral, and staff researchers, clinical investigators, and principal investigators to provide quality customer service.
Maintain accurate, complete, up-to-date notes on experimental details and results using an electronic laboratory notebook, and project and task management applications.
Work closely with the Research Associate I as part of a team to fulfill service requests to meet quality standards and stated turnaround times.
Liaise with bioinformatics team to check the quality of sequenced samples.
Follow manufacturer’s protocols and core-specific workflows to ensure generation of high quality results.

Education:

Associate Degree in biology, biochemistry, or related science/engineering field is required. Bachelor's degree is preferred.

Experience & Skills:

One (1) year of experience working in a lab or biotech environment.
One (1) year of experience working in an aseptic environment, experience working in a biosafety cabinet, and experience working with human and animal tissues.
One (1) year of experience maintaining and/or using biomedical equipment for testing.
Collaborates to problem solve and make decisions to achieve desired outcomes.
Responds timely, effectively and appropriately to deliverables.
Establishes effective working relationships with cross-functional team(s).

Working Title: Biomedical Technician - Applied Genomics, Computation & Translational Core
Department: BMS - Bioinfo & Func Genomics
Business Entity: Cedars-Sinai Medical Center
Job Category: Biomanufacturing
Job Specialty: Biomanufacturing
Position Type: Full-time
Shift Length: 8 hour shift
Shift Type: Day
Base Pay:$29.00 - $44.95

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About Cedars-Sinai Medical Center

Providing healthcare for more than 100 years, Cedars-Sinai has evolved into one of the most dynamic and highly renowned medical centers in the world. Along with caring for patients, Cedars-Sinai is a hub for biomedical research and a training center for future physicians and other healthcare professionals. This attracts exceptional talent to Cedars-Sinai, including world-renowned physician-scientists who seek a place where they can both conduct research and see patients--the ideal formula for discovery and its translation into cures. Our patients benefit from access to doctors at the top of their fields, and our researchers have an ideal community in which to study the impact of healthcare challenges, and reflect that knowledge in their research. The greater Los Angeles area in which Cedars-Sinai resides possesses unparalleled cultural and ethnic diversity which offers outstanding oppor...tunities for translational and clinical research and a dynamic environment for medical education.Although community based, Cedars-Sinai is a major teaching hospital affiliated with the David Geffen School of Medicine at the University of California, Los Angeles (UCLA). Cedars-Sinai has highly competitive graduate medical education programs in more than 50 specialty and subspecialty areas, a graduate program in biomedical sciences and translational medicine, a clinical scholars program directed towards junior physicians with aspirations to become clinical scientists, and post graduate training opportunities.There are more than 250 full-time faculty members at Cedars-Sinai. The voluntary medical staff, comprised of more than 2,200 specialty board-certified or board-qualified physicians, represent all of the specialties and subspecialties and collaborate with full-time medical staff in the teaching responsibilities of the graduate medical education programs.

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