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Mouse Researcher in Women's Infectious Diseases

University of California San Diego

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Application

Details

Posted: 14-Apr-22

Location: La Jolla, California

Salary: Open

Internal Number: 115737

UCSD Layoff from Career Appointment: Apply by 04/15/22 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.

Special Selection Applicants: Apply by 04/27/22. Eligible Special Selection clients should contact their Disability Counselor for assistance.

Depending on experience of the selected candidate, this position will be hired at the SRA 2 NEX or SRA 3 level.

DESCRIPTION

The UCSD Department of Obstetrics, Gynecology, and Reproductive Sciences strives to provide excellent clinical care to women while conducting cutting-edge basic, translational, and clinical research in reproductive health.

The Staff Research Associate will participate in an integrated research team focused on cutting edge research related to how the vaginal microbiome interacts with its host to produce outcomes of health and disease. The Staff Research Associate will independently design and carry out preclinical studies employing mouse infection/inoculation models. Apply existing skills to make important contributions to the experimental mouse models component of our research team. Work with mouse handling and basic routes of administration; willingness to learn new routes of administration and procedures. Projects may involve timed mating, genotyping, establishment/phenotyping of transgenic lines, histology. Manage in microbiology/microbiome protocols and analysis, molecular biology (bacterial genetics, qRT-PCR, plasmid construction, RNAseq etc), biochemistry (chromatography, enzyme assays, heterologous protein expression etc), flow cytometry.

MINIMUM QUALIFICATIONS

• Theoretical knowledge of general biology, biochemistry, cell biology, and molecular biology, or related field as typically attained through a Bachelor's degree and/or an equivalent combination of education and experience.

• Strong experience working with a variety of laboratory equipment, including but not limited to equipment used with animals.

• Willingness to perform (and at least five (5) years of experience in) experiments involving the handling, dissection, and sacrifice of laboratory mice. Ability to work with recombinant DNA, biohazardous and infectious materials, and toxic chemicals.

• Demonstrated strong success and skill at researching, analyzing and evaluating information for preparation of grant proposals, manuscripts, and communications.

• Proven ability to work independently and in collaboration with the Principal Investigator on research projects, organize, prioritize, and meet critical deadlines for multiple on-going research and development projects.

• Knowledge of laboratory safety including radioactive/biohazard handling and disposal techniques, and the ability to ensure laboratory compliance with required safety regulations.

• Demonstrated experience with growing cells and cells in culture using sterile/asceptic techniques.

• Strong experience with DNA isolation, PCR and gel electrophoresis (required)., DNA digestion by restriction enzymes, ligation, plasmid transformation in bacteria, quantitative real time PCR, RNA isolation, cDNA preparation, Sanger sequencing, additional sophisticated molecular biology tools (any of these skills a plus).

• Excellent interpersonal skills to be effective in communicating and interacting with a variety of people within a large laboratory setting and in administrative components within and outside the University.

• Knowledge of molecular biology, recombinant DNA and occasionally with toxic materials and radioactive isotopes.

• Strong computer skills and experience with basic software packages including Microsoft Word, Excel, and PowerPoint (required). Experience with and/or willingness to learn graphing programs, drawing programs, and other bioinformatics or statistical analysis software would be a plus.

• Proven ability to handle, inject, euthanize, and collect tissues from mice.

• Demonstrated experience in maintaining well-organized lab and thorough records. Basic math skills and ability to handle accounting and billing practices.

• Ability to work with tissue culture cells under sterile conditions.

• Proven knowledge of small animal surgery and anatomy, mouse husbandry, and reproductive functions.

PREFERRED QUALIFICATIONS

• Demonstrated superior oral and written communication skills to communicate research results and carry out collaborative projects
• Experience or knowledge in other core approaches/areas of the lab, such as molecular biology (especially qPCR), biochemistry (especially chromatography), reproductive biology, or carbohydrate biology.

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About University of California San Diego

UC San Diego is an academic powerhouse and economic engine, recognized as one of the top 8 public universities by U.S. News and World Report. Innovation is central to who we are and what we do. Here, students learn that knowledge isn't just acquired in the classroom - life is their laboratory. UC San Diego's rich academic portfolio includes six undergraduate colleges, five academic divisions and five graduate and professional schools. The university's award-winning scholars are experts at the forefront of their fields with an impressive track record for achieving scientific, medical and technological breakthroughs.

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